Journal: Journal of Translational Medicine

Article Title: Spatiotemporal microenvironment landscape and malignant epithelial pattern transition in breast ductal carcinoma progression

doi: 10.1186/s12967-025-07010-3

Figure Lengend Snippet: Lineage and tissue distribution heterogeneity of T lymphocytes. A : Tsne plot depicting T/NK cell populations in normal tissue, DCIS, IDC, and LM. B : Relative enrichment of different T/NK subsets across various sampling regions. C : Similarity tree constructed based on the top 50 expressed genes of T cell subsets. D : Weighted heatmap of cell-cell communication interactions among T/NK subsets. E : Developmental trajectory of CD4 T cells, showing distinct cell types (left) and differentiation potential (right). F : Developmental trajectory of Treg cells (left) and bar plot of the percentage of cells in five states (right). G : Heatmap displaying the average expression of nTreg marker genes across the five Treg states. H : Relative enrichment of nTreg and iTreg in different sampling regions. I : Developmental trajectory of CD8 T cells, showing distinct cell types (left) and differentiation potential (right). J : Scatter plot of differentially expressed genes between CD8 T cells in IDC and lymph node metastasis. K : Box plot showing differences in T cell exhaustion and costimulation scores of CD8 T cells across various sampling regions. L : Proportional differences of T cell subsets in normal mammary tissue, DCIS, and IDC. M : mIHC sections revealing the distribution of FOXP3 + CD25 + nTreg cells in IDC and lymph node metastasis.

Article Snippet: All primary antibodies include FOXP3 (bs-10211R, BIOSS), IL2RA (af5105, AFFINITY), CD68 (ba3638, BOSTER), and EGR1 (4153s, CST).

Techniques: Sampling, Construct, Expressing, Marker